Cell Line

Evidence that gene activation and silencing during stem cell differentiation requires a transcriptionally paused intermediate state

A surprising portion of both mammalian and Drosophila genomes are transcriptionally paused, undergoing initiation without elongation. We tested the hypothesis that transcriptional pausing is an obligate transition state between definitive activation …

High levels of transgene expression following transduction of long-term NOD/SCID-repopulating human cells with a modified lentiviral vector

Both oncoretroviral and lentiviral vectors have been shown to transduce CD34(+) human hematopoietic stem cells (HSC) capable of establishing human hematopoiesis in nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice that support …